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Immunomagnetic Separation
Immunomagnetic separation techniques for CTC enrichment depend on the expression of epithelial-specific and/or tumor-associated protein markers discussed in Section IIC For example, MACS (Miltenyi Biotec, Bergisch Gladbach, Germany) utilizes a magnetic column apparatus that captures cells which have been bound by magnet bead-conjugated ....
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Cell Isolation
Immuno-magnetic separation of cells is based on the deflection of cells in a magnetic gradient field The magnetic properties of the cells can be intrinsic, eg iron containing RBCs or on account of superparamagnetic particles coated with antibodies directed against specific antigens...
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Healthy cells in = good data out
products that do not adversely affect cells during isolation Dynabeads® magnetic separation technology can be used to isolate pure, viable, and functional cells of the immune system to advance your immunology research With this technology, your cells are not exposed to the stress...
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Immune Cell Function
The Immune Cell Function assay measures the increase in intracellular ATP production that occurs in T-lymphocytes within 24 hours of stimulation by antigens or mitogens 3 , ATP measurement appears to be a useful clinical indicator of cell-mediated immune , Magnetic separation ,...
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Magnetic particle separation : a short review
Commercial magnetic particle separation One of the earliest works in magnetic capturing using bulk magnets was reported by Miltenyi in [1] In this work, a Magnetic Cell Sorter (MACS) from Miltenyi Biotec is used to separate cells labeled with magnetic particles from non-labeled cells...
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MAGNETIC SEPARATION OF CELLS
Sep 25, 2014· An apparatus, system, and method for magnetic separation of cells are disclosed By combining inkjet printing technology and magnetic labeling of cells, accurate cell counts are obtained using an optical microscope Mouse CD4+ lymphocytes are attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer...
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Magnetic micro
Dec 23, 2015· Liu Y, CheY, Li Y (2001) Rapid detection of Salmonella typhimurium using immunomagnetic separation and immune-optical sensing methodSensActuat B72: 214-218 Guo PL, Tang M, Hong SL, Yu X, Pang DW, et al (2015) Combination of dynamic magnetophoretic separation and stationary magnetic trap for highly sensitive and selective detection of ....
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CD8 MicroBeads, human
Our lab routinely works with purified immune cell subsets from human peripheral blood mononuclear cells (PBMC) In order to obtain highly pure CD8+ T cells, as well as other immune cell types, we routinely employ magnetic separation with Miltenyi products for positive selection because of their ease of use and minimal optimization...
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A Modified Lentivirus
Cancer stem cells (CSCs) are the most malignant subpopulation of tumor cells that possess a tumorigenic potential and resistantance to chemotherapy These properties make CSCs a promising target for the development of targeted antitumor therapy which is especially in demand in highly aggressive cancers However, the correct identification of cancer cells with stem properties remains a ,...
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Detection of Circulating Endothelial Cells: CD146
Sep 21, 2016· It is undisputed that CD146-based magnetic separation rarely provides an absolutely pure fraction of endothelial cells and that the procedure ,...
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Immunomagnetic separation
Magnetic cell separation, also known as immunomagnetic cell separation, involves targeting cells for selection or depletion using antibodies or ligands directed against specific cell surface antigens Labeled cells are cross-linked to magnetic particles, also known as magnetic beads, that can be immobilized once an electromagnetic field is applied...
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Immunospecific ferromagnetic iron
The high-gradient magnetic separation tech- nique used by Melville et al (1975) and Owens (1978) to remove paramag- netically induced erythrocytes from blood should be particularly valuable for the efficient, preparative magnetic separation of cells, cell membranes or receptors specifically labeled with ferromagnetic iromdextran particl...
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A Simple Magnetic Separation Method for High
Jan 15, 2011· With the advent of highly specific monoclonal antibodies that label unique cell populations, magnetic cell separation technology has progressed to become an indispensable tool for cell separation in the field of immunology, and has been widely used to separate and analyze almost every immune cell type (Grutzkau and Radbruch, 2010)...
